Project website :
Partners of the project :
Links to the .ppt from Faro meeting:
Project summary :
Despite the impact that viruses have on aquatic organisms, relatively little is known about what farmers can do to prevent and treat viral diseases and how fish and invertebrates fight these infections. Thus, the project aims to characterise anti-viral innate immunity in fish, bivalves and crustaceans in order to provide new approach for the control of viral infections in aquaculture species. Some molecules and genes involved in anti-viral innate inhumanity will be defined and compared in different species. Selected anti-viral effectors will be monitored in vitro and in vivo assays potential anti-viral effects in different cellular and animal models. Furthermore, expression of selected molecules encoding genes will be analysed in healthy and virus-infected individuals. Candidate molecules and genes will be supplied for further exploitation in aquaculture and development of control tools against human and animal viral diseases.
List of genomic tools generated in the project :
Detection and biochemical characterisation of anti-viral molecules. Despite a cytotoxicity detected, a putative antiviral substance has been detected in Pacific oyster haemolymph. No antiviral activity was detected in crustacean (crab and shrimp) heamolymph as in sea bream and carp sera and tissue extracts. High cytotoxicity was observed in these samples.
Identification of virus-induced genes. RNAs have been extracted from sea bream macrophages, carp leucocytes from head kidney and oyster hemocytes after virus contact. The expected achievement of this work package is to characterize new genes involved in anti-viral innate immunity in different aquatic species. The Suppression Subtractive Hybridization technique (SSH) is used to carry out this step and promissing candidate genes have been obtained in the different models.
Molecular characterisation of cellular IAPs. Attemps to detect survin RNAs were unsuccessful in two tested fish species (sea bream and common carp). Research targeting genes related to mammalian IAP-1 and IAP-2 have been successful: a single IAP gene and two dffferent genes were identified in sea bream and common carp, respectively.
Publications generated in the project :