Project website : fPPARS website
Partners of the project :
Links to the .ppt from Faro meeting:
Project summary :
The specific objectives of this project are:
1) to clone and characterise the PPAR genes and cDNAs from four fish species of particular importance to the European aquaculture industry, i.e. sea bass (Dicentrarchus labrax), sea bream (Sparus aurata), Atlantic salmon (Salmo salar), and plaice (Pleuronectes platesa)
2) to study the functional properties of fish PPARs (fPPARs), i.e. their DNA and ligand binding properties, the PPAR-dependent transcriptional activation, and the expression pattern of PPARs in fish tissues; to establish species-specific and PPAR subtype-specific differences in the above described properties; to identify potent subtype-specific ligands for the fPPARs
3) to develop appropriate in vitro assays, i.e. cell and tissue culture systems, that will be used to establish clearly the role of PPARs in lipid metabolism in fish.
4) to examine in vivo the effect of induced PPAR expression and PPAR-dependent transcriptional activation on fat accumulation and lipid composition in fish tissues.
List of genomic tools generated in the project :
1) the cloning and sequence analysis of three PPAR subtypes from sea bass, sea bream, Atlantic salmon, and plaice
2) the development of fish PPAR (fPPAR) subtype-specific antibodies, used in the study of the functional properties of the fPPARs
3) protocols have been established for the primary culture of hepatocytes, adipocytes, and enterocytes from the fish species studied. These primary cell culture systems will provide a valuable tool for the in cellulo study of the fPPAR properties.
The above results will allow the study of the properties of fPPARs both in vitro and in vivo. Concerning the functional properties of the fPPARs, i.e. transcription activation properties, tissue distribution, and DNA and ligand binding properties, preliminary results support the hypothesis that these receptors have functions similar to those of their mammalian homologues and are, therefore, key regulators of lipid homeostasis in fish.
Publications generated in the project :
1. Boukouvala E, Antonopoulou E, Favre-Krey L, Diez A, Bautista JM, Leaver MJ, Tocher DR, Krey G (2004) Molecular characterization of three peroxisome proliferators-activated receptors from the sea bass (Dicentrarchus labrax). Lipids, 39, 1085-1092.
2. Leaver MJ, Boukouvala E, Antonopoulou E, Diez A, Favre-Krey L, Ezaz MT, Bautista JM, Tocher DR, Krey G (2005) Three peroxisome proliferators-activated receptor isotypes from each of two species of marine fish. Endocrinology, 146, 3150- 3162.
3. Diez A, Pérez-Benavente S, Calduch-Giner JA, Vega-Rubin de Celis S, Obach A, Favre-Krey L, Boukouvala E, Menoyo D, Leaver MJ, Tocher DR, Pérez-Sanchez J, Krey G, Bautista JM. Dietary conjugated linoleic acid supplementation modifies transcription of peroxisome proliferators-activated receptor isotypes in sea bream: effects on lipid metabolism and endocrine control (submitted).
4. Leaver MJ, Tocher DR, Obach A, Jensen L, Henderson RJ, Porter A, Krey G. Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (in preparation).
5. Leaver MJ, Ezaz MT, Fontagne S, Tocher DR, Boukouvala E, Krey G. Multiple peroxisome proliferator-activated receptor β subtypes from Atlantic Salmon: Gene identification, tissue expression and functional characterization (in preparation).